STUDY ON THE ANTAGONISTIC ACTIVITY AGAINST TWO PATHOGENIC BACTERIA IN HUMANS OF STREPTOMYCES MIP_L26 ISOLATED FROM SOIL IN THE ROOT ZONE OF THE MAY CHANG TREE (LITSEA CUBEB)
DOI:
https://doi.org/10.59459/1859-1655/JMM.377Từ khóa:
Streptomyces MIP_L26, May Chang tree (Litsea cubeba), antagonistic activity, 16S rRNATóm tắt
Purpose: Study on the antagonistic activity against two pathogenic bacteria in humans (Staphylococcus aureus and Streptococcus pneumoniae) of the Streptomyces MIP_L26 (Called MIP_L26), isolated from the soil in the root zone of the May Chang tree; to investigate the biological characteristics of MIP_L26
Subjects and methods: Experimental study, determine the antimicrobial activity against two pathogenic bacteria in humans (Staphylococcus aureus and Streptococcus pneumoniae), and some biological characteristics of MIP_L26.
Results: MIP_L26 was identified as having a strong antagonistic ability against the two bacteria causing Staphylococcus aureus and Streptococcus pneumoniae with an antibacterial ring diameter of 18 ± 5 mm and 13 ± 5 mm, respectively. Based on classification according to morphological, physiological, and biochemical characteristics, combined with the results of 16S rRNA gene sequencing, MIP_L26 was identified as Streptomyces gancidicus MIP_L26. The fermentation medium for the biosynthesis of antibacterial substances was ISP6 and the recovery time for antimicrobial substances was after 72 hours of fermentation.
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Chấp nhận đăng 13-12-2023
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